Regulation of pathogenesis-related protein-1a gene expression in tobacco.
نویسندگان
چکیده
منابع مشابه
Regulation of Pathogenesis-Related Gene Expression in Tobacco
Pathogenesis-related protein-la (PR-la) is a protein of unknown function that is strongly induced during the onset of systemic acquired resistance (SAR) in tobacco. The expression of PR-la is under complex regulation that is controlled at least partially by the rate of transcription. In this study, we demonstrated that 661 bp of 5’flanking DNA was sufficient to impart tobacco mosaic virus and s...
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The cis-acting elements for regulating gene expression of the tobacco pathogenesis-related 1a protein gene were analyzed in transgenic plants. The 5'-flanking 2.4-kilobase fragment from the pathogenesis-related 1a protein gene was joined to the bacterial beta-glucuronidase gene and introduced into tobacco cells by Agrobacterium-mediated gene transfer. Promoter activity was monitored by quantita...
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Acetolactate synthase (ALS) catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine. The previous cloning of two tobacco (Nicotiana tabacum) ALS genes (SurA and SurB) has allowed transcript accumulation from these genes to be monitored. mRNA blot analysis of ALS transcripts showed a message size of 2.2 kb. Quantitation of the levels of ALS messages in tobacco orga...
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متن کاملThe nucleotide sequence of pathogenesis-related (PR) 1b protein gene of tobacco
A genomic clone encoding the pathogenesis-related (PR) lb protein was cloned from Nicotiana tabacum cv. Samsun NN genomic library screening with cDNA clone of PRla protein gene (1). The following nucleotide sequence contains coding and its flanking regions. The nucleotide sequence of PRlb protein gene is highly similar to that of PRla (2-5). Coding region is from 457 to 963 without intron and c...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1993
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.5.2.159